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| 产品名称： | pCD11A |
|---|---|
| 商品货号： | TS185567 |
| Designations： | pCD11A |
| GenBank Number： | M19503 |
| Species： | Homo sapiens, human |
| Depositors： | MF Singer, RE Thayer |
| Vector： | Construct size (kb): 6.099999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.394999980926514 Tissue: teratocarcinoma NTera2D1 cell line Gene product: LINE consensus sequence (KpnI sequence), repetitive |
| Insert Size (kb)： | 3.395 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.4, 2.7; KpnI--4.2, 1.9; BglII--4.4, 1.7; HindIII--4.2, 1.9; BglI--4.6, 1.4. The insert contains the following restriction sites (nt position according to the published sequence): KpnI--65, 1862; BglII--630, HindIII--1734. Contains nt 1-3395 of the published sequence, including a 5 leader, ORF1 (nt 769-1889), the inter-ORF region, and the 5 end of ORF2 (nt 1929-5781). At the 5 end, this sequence falls short of being a complete copy of the cDNA by 32 bp. |
| References： | Skowronski J, et al. Unit-length Line-1 transcripts in human teratocarcinoma cells. Mol. Cell. Biol. 8: 1385-1397, 1988. PubMed: 2454389 |