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微信小章| 产品名称： | pRat alpha 2 |
|---|---|
| 商品货号： | TS185626 |
| Designations： | pRat alpha 2 |
| GenBank Number： | M14512 |
| Species： | Rattus norvegicus, rat |
| Depositors： | JB Lingrel |
| Applications： | isoform-specific probe ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide |
| Vector： | Construct size (kb): 3.16700005531311 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC18 Intact vector size: 2.686 Type of vector: plasmid Vector end: SmaI Vector end: SmaI Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Construction: pUC71K Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ, MCS: HindIII...EcoRI, -> promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: DNA Seq. Acc.: L08752 |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: rat Gene symbol: ATP1A2 Gene name: ATPase, Na+/K+ transporting, alpha 2 polypeptide Contains complete coding sequence?: N Strain: CD Tissue: brain Type of DNA: cDNA Insert 5 end: ScaI Insert 3 end: NheI Modification: blunted Insert size (kb): 0.381 Cross references: DNA Seq. Acc.: M14512 Nucleotides 1-381 of the insert correspond to nucleotides 121-502 of M14512. Insert lengths(kb): 0.3810000121593475 Tissue: brain Gene product: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide ATP1A2 Target Gene: ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide |
| Insert Size (kb)： | 0.381 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | This is a 5 specific probe for the alpha2 isolform. It is not full length. Nucleotides 1-381 of the insert correspond to nucleotides 121-502 of Genbank M14512. The insert contains the following restricti sites (approximate kb from the 5 end): SacI--0.06, 0.17; NcoI--0.09, 0.33. Restriction digests of the clone give the following sizes (kb): EcoRI/PstI--2.7, 0.4; BglI--2.0, 1.2; PvuII--2.4, 0.7. |
| References： | Young RM, Lingrel JB. Tissue distribution of mRNAs encoding the alpha isoforms and beta subunit of Rat Na+, K+-ATPase. Biochem. Biophys. Res. Commun. 145: 52-58, 1987. PubMed: 3036133 Orlowski J, Lingrel JB. Tissue-specific and developmental regulation of Rat Na,K-ATPase catalytic alpha isoform and beta subunit mRNAs. J. Biol. Chem. 263: 10436-10442, 1988. PubMed: 2839491 Shull GE, et al. Molecular cloning of three distinct forms of the Na+,K+-ATPase alpha subunit from Rat brain. Biochemistry 25: 8125-8132, 1986. PubMed: 3028470 |