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| 产品名称： | pPAI-E6.9 |
|---|---|
| 商品货号： | TS185676 |
| Designations： | pPAI-E6.9 |
| GenBank Number： | J03764 |
| Species： | Homo sapiens, human |
| Depositors： | PJ Bosma |
| Vector： | Construct size (kb): 11.0 |
| Insert： | DNA: genomic Insert lengths(kb): 6.9 Gene product: plasminogen activator inhibitor, type I PAI1 |
| Insert Size (kb)： | 6.900 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Among the restriction sites in the insert are the following: PstI (11), SmaI (4), NcoI (2), DraI (2), NarI (2), MstII (3), PvuII (3), BglI (1), TaqI (4) and NaeI (2). Restriction digests of the clone give the following sizes (kb): EcoRI--6.4, 2.8; BamHI--11.0; HindIII--11.0; PstI--4.2, 1.9 doublet, 1.1, 0.6, 0.58; BglI--7.9, 2.7; BamHI/EcoRI--7.4, 1.7, 1.2. The insert includes part of intron 1 through part of intron 7. This sequence includes multiple Alu repeats, two purine-rich regions, and a Z-DNA structured region. |
| References： | Bosma PJ, et al. Human plasminogen activator inhibitor-1 gene. Promoter and structural gene nucleotide sequences. J. Biol. Chem. 263: 9129-9141, 1988. PubMed: 3132455 |