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| 产品名称： | pPL603 |
|---|---|
| 商品货号： | TS185712 |
| Designations： | pPL603 |
| Depositors： | PS Lovett |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 4.6999998092651370 Vector: pPL603 (plasmid) Promoters: Promoter none Construction: pUB110, Bacillus pumilis cml Marker(s):kanR,neoR Construct size (kb): 4.699999809265137 Features: insert detection: CAT marker(s): kanR, neoR promoter: none replicon: pUB110 terminator: none enhancer: none |
| Applications： | promoter-cloning vector shuttle vector |
| Comments： | Cat-86 is not expressed until a promoter-containing fragment is cloned into the unique EcoRI site (350 bp upstream). A promoter-cloning vector for Bacillus subtilis. |
| Media： | ATCC® Medium 1179: VY medium |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Williams DM, et al. Cloning restriction fragments that promote expression of a gene in Bacillus subtilis. J. Bacteriol. 146: 1162-1165, 1981. PubMed: 6787015 Duvall EJ, et al. Chloramphenicol-inducible gene expression in Bacillus subtilis. Gene 24: 171-177, 1983. PubMed: 6416927 |
| Shipped： | freeze-dried |