Construct size (kb): 5.199999809265137

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, insert detection: lacZ, primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, promoter: lac, replicon: pMB1, marker(s): ampR, Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PPAR
Genomic copy number: unique
Gene name: peroxisome proliferator activated receptor
Contains complete coding sequence?: Y
Chromosome: 22; Localization: 22 q12-q13.1
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 2.1
Cross references: DNA Seq. Acc.: L07592; EST: EST06159
Nucleotides 1-359 of the insert correspond to
nucleotides 243-601 of L07592 with 97% identity.
DNA Seq. Acc.: T08268; EST: EST06159; Primer: M13 Reverse;
THC id: THC97583

Insert lengths(kb): 2.099999904632568

Tissue: infant brain, 3 months old (from Bento Soares)

Gene product: peroxisome proliferator activated receptor PPAR

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): EcoRI--5.2; HindIII+NotI--3.2, 2.08.