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微信小章| 产品名称： | Human adenovirus 10 |
|---|---|
| 商品货号： | TS185811 |
| Classification： | Adenoviridae, Mastadenovirus, Human mastadenovirus D |
| Agent： | Human adenovirus 10 |
| Strain： | Trim-ATCC |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | This product was derived from ATCC VR-1604 ™ by three rounds of plaque purification. |
| Effect on Host： | CPE, cell rounding and sloughing |
| Recommended Host： | A-549 cells (ATCC CCL-185) |
| Growth Conditions： | Temperature: 37°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation:xa01-7 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor： | ATCC, NIAID |
| Source： | Presumed to be from a case of epidemic keratoconjunctivitis, California, 1955. |
| References： | Jawetz E,Kimura S, Nicholas AN, Thygeson P and Hanna L. new type of APC virus from epidemic keratoconjunctivitis. Science 122: 1190-1191, 1955. PubMed: 13274081 Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904 Elnifro EM et al. PCR and restriction endonuclease analysis for rapid identification of human adenovirus subgenera. J. Clin. Microbiol. 38(6): 2055-2061, 2000. PubMed: 10834953 |