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微信小章| 产品名称： | Cryptococcus neoformans var. grubii Franzot et al. |
|---|---|
| 商品货号： | TS185827 |
| Deposited As： | Cryptococcus neoformans (Sanfelice) Vuillemin |
| Strain Designations： | H99 H99JP, NYSD 1649, CBS8710 |
| Application： | Biomedical Research and Development Material
Emerging infectious disease research
Negative control in differential gene expression studies
Produces laccase |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | yes |
| Antigenic Properties： | Serotype A |
| Genotype： | VNI |
| Preceptrol®： | no |
| Genome Sequenced Strain： | Yes |
| Mating Type： | alpha (Peter R Williamson, personal communication) |
| Comments： | Multigene phylogeny and phenotypic characterization.xa0
Facultative intracellular pathogenxa0 Genome sequencing strain (Broad Institute, USA) Designated as type strain of Cryptococcus neoformans (Sanfelice) Vuillemin by Hagen et al. (2015) |
| Morphology： | After 8 days on YM agar at 30°C, colony is cream-colored, smooth, mucoid. Cells globose or subglobose, single or with bud, pseudohyphae not observed. |
| Medium： | ATCC® Medium 28: Emmons modification of Sabourauds agar ATCC® Medium 200: YM agar or YM broth ATCC® Medium 1245: YEPD |
| Growth Conditions： | Temperature: 25°C to 30°C Atmosphere: Typical aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence GGTTTCCGTAGGTGAACCTGCGGAAGGATCAGTAGAGAATATTGGACTTCGGTCCATTTATCTACCCATCTACACCTGTGAACTGTTTATGTGCTTCGGCACGTTTTACACAAACTTCTAAATGTAATGAATGTAATCTTATTATAACAATAATAAAACTTTCAACAACGGATCTCTTGGCTTCCACATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCAACTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGAGTCATGAAAATCTCAATCCCTCGGGTTTTATTACCTGTTGGACTTGGATTTGGGTGTTTGCCGCGACCTGCAAAGGACGTCGGCTCGCCTTAAATGTGTTAGTGGGAAGGTGATTACCTGTCAGCCCGGCGTAATAAGTTTCGCTGGGCCTATGGGGTAGTCTTCGGCTTGCTGATAACAACCATCTCTTTTTGTTTGACCTCAAATCAGGTAGGGCTACCCGCTGAACTTAAGCATATCAATAA D1D2 region of the 28S ribosomal RNA gene. ATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGTGAACCGGGAAGAGCTCAAATTTGAAATCTGGCGTCCTCCGGGCGTCCGAGTTGTAATCTACAGAAACGTTTTCCGTGCTGGACCGTGTCTAAGTCCCTTGGAATAGGGTATCAAAGAGGGTGACAATCCCGTACTTGACACGATCACCAGTGCTCTGTGATACGTTTTCTACGAGTCGCGTTACTTGGGAGTGTAGCGCAAAATGGGTGGTAAACTCCATCTAAAGCTAAATATTGGTGGAAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAACGATTGAAGTCAGTCGTGTCTATTGGGTTCAGCCAGTTCTGCTGGTGTATTCCCTTTAGACGGGTCAACATCAGTTCTGATCGGTGGATAAGGGCTGGGGGAATGTAGCACTCTTCGGAGTGTGTTATAGCCTCCTGTCGCATACACTGGTTGGGACTGAGGAATGCAGCTCGCCTTTATGGCCGGGGTTCGCCCACGTTCGAGCTTAGGATGTTGACAAAATGGCTTTAAACGAC 28S ribosomal RNA gene and intergenic spacer (IGS), partial sequence ATGGGAACGGGGTGCTGTAAGTGGTAGAGTAGCCTTGTTGCTACGATCCACTGAGGCTAAGCCCTTGTTCTATAGATTTGTCTCTAACATGTTGGGTCTCGGGGGGCTTCCTCTAGAGACTTGGATGTAAGGGGCTTTACGCATTCGCATAACTTATGAAGTGTGGATGACTCTAGGAGGGGTCTGAGAATGTGCTGTGCCAGCCAGGCTGATAATAGTTTAATTGTTAGCTTGGACTTGTACACAGTCTCATCAGTCTTCAGCTTGGGTCATTGATATTCTGTAAGTCAAAACTTATCCATTCACTGTAGTAGGTTGCGGAGGACTTGAAAGTTCTCTCTGCAATTGAGACACTTACCAGGCCATCGCAAGTTGGCAGTGTACGCTACTGATTTTATGGTCGTGGGGGACTTGGGAGCTGGTGCTTGTGTCGCATGAGGTAGAGTTGATATGGACGGATGGAAGAGAAGCATATGTGTATGTCGCGGGGGACTTGGGGTAAGACGCCTTGCGGCAAGTAGAGTCAACCAGTAGTGACTTCTTTTAGTCGTGGGGGACTTTGGTAATGGTAGTGAGCCGGAATAAGGGATGGATAAATGTAGTATGGATGGTGACAGGGGGAGCAGCAGAGCGTACGCAGTGGGTATGTATATAGTTGAAAAGGGATGGGCAGTAGAATTTTGAGAAATTAATGACCTAAGGGTGATTCTAATAGGCTTGTCAAGAAAAAAGTTAAGTTGAATCAAGCTGGGTCAAGCAAAGTCTAGAAAAGATTTATTGTCACCAATAATTGCCCAAGTCGCGGGGGGCTTGTCACTTCTCTTGCTCATTGTGGGTCCAGTCT glycerol-3-phosphate dehydrogenase 1 (GPD1) gene, partial cds TACTTGCATAAATACAATGGTCGTCAAGGTTGGAATCAACGGTTTCGGTACGTAATCGATCCCTCTCTTCCCATTGCTTCATATACCACCGATGTGACTTCCGAATTTGTGCGCTGTTCTTGAGCTACGGTAGCTAACAACTATGTGATAGGTCGTATTGGTCGAATTGTTCTCAGGTATGCTTTTAAATCAACGGGGTAGGACGTCCATGTATCTAACATTCAAGTTAGGAACGCCATCGAGCATGGAGATCTCGAGGTTGTTGCTGTCAACGAGTGAGTGTCCCAAATGTTGACCCATTGGATGAATTACTAAATTGTCCATAGCCCTTTCATTGACTTGGACTACATGGTACGGTACTCCTGTCTGCCCAATAACCTCGTTTAGTACTAACGCTATTGCACAGGTCTACATGTTCAAGTATGACTCCGTAAGTCTTCGATACTTCTAGCGCTCTATATGGCTTATTAACCTGATTCCCGACAGACTCATGGTCGTTTTAAGGGCTCCGTCGAGGTTAAGGACGGTAAGCTTTACATCAACAACAAGGCCATTGCCGTCTTCGGTGAGAAGGACCCTGCCAACATCAAGTGGGGTGAGGCTGGTGCCGAATACGTCGTTGAATCTACCGGTGTTTTCACGACTACCGAAAAGGCCAGTGTTCAT |
| Name of Depositor： | PR Williamson |
| Special Collection： | NCRR Contract |
| Chain of Custody： | ATCC |
| Isolation： | Patient with Hodgkins disease, New York |
| Cross References： | Nucleotide (GenBank) : KU729082 ITS including 5.8S rRNA gene Nucleotide (GenBank) : AF254790 Filobasidiella neoformans var. neoformans vacuolar (H+)-ATPase Nucleotide (GenBank) : AF098972 phosphatidylinositol 3-kinase TOR1 (TOR1), complete coding sequence Nucleotide (GenBank) : AF097888 macrolide-binding protein FKBP12 (FRR1) gene, complete coding sequence Nucleotide (GenBank) : AF097889 macrolide-binding protein FKBP12 (FRR1) mRNA, complete coding sequence Nucleotide (GenBank) : AACO02000000 Cryptococcus neoformans var. grubii H99, whole genome shotgun sequencing project. |
| References： | Williamson PR, et al. Melanin biosynthesis in Cryptococcus neoformans. J. Bacteriol. 180: 1570-1572, 1998. PubMed: 9515929 Feldmesser M, et al. Cryptococcus neoformans is a facultative intracellular pathogen in murine pulmonary infection. Infect. Immun. 68: 4225-4237, 2000. PubMed: 10858240 del Poeta M, et al. Cryptococcus neoformans differential gene expression detected in vitro and in vivo with green fluorescent protein. Infect. Immun. 67: 1812-1820, 1999. PubMed: 10085022 Franzot SP, et al. Cryptococcus neoformans var. grubii: separate varietal status for Cryptococcus neoformans serotype A isolates. J. Clin. Microbiol. 37: 838-840, 1999. PubMed: 9986871 Cruz MC, et al. Rapamycin antifungal action is mediated via conserved complexes with FKBP12 and TOR kinase homologs in Cryptococcus neoformans. Mol. Cell. Biol. 19: 4101-4112, 1999. PubMed: 10330150 Heitman J, et al. The Cryptococcus neoformans genome sequencing project. Mycopathologia 148: 1-7, 1999. Peter R Williamson, personal communication Findley K, et al. Phylogeny and phenotypic characterization of pathogenic Cryptococcus species and closely related saprobic taxa in the Tremellales. Eukaryot Cell 8(3): 353-361, 2009. PubMed: 19151324 Wang H, et al. A fungal phylogeny based on 82 complete genomes using the composition vector method. BMC Evol. Biol. 9: 195, 2009. PubMed: 19664262 Ding C, et al. The copper regulon of the human fungal pathogen Cryptococcus neoformans H99. Mol. Microbiol. 81: 1560-1576, 2011. PubMed: 21819456 Meyer W. et al. Consensus multi-locus sequence typing scheme for Cryptococcus neoformans and Cryptococcus gattii. Med. Mycol. 47(6):561-570, 2009. PubMed: 19462334 Urai M, et al. Evasion of innate immune responses by the highly virulent Cryptococcus gattii by altering capsule glucuronoxylomannan structure. Front Cell Infect Microbiol 5: 101. doi: 10.3389/fcimb.2015.00101. PubMed: 26779451 Sorrell TC, et al. Cryptococcal transmigration across a model brain blood-barrier: evidence of the Trojan horse mechanism and differences between Cryptococcus neoformans var. grubii strain H99 and Cryptococcus gattii strain R265. Microbes Infect 18: 57-67, 2016. PubMed: 26369713 Movahed E, et al. Genome-wide transcription study of Cryptococcus neoformans H99 clinical strain versus environmental strains. PLoS One 10: e0137457, 2015. PubMed: 26360021 Chang YC, et al. Differences between Cryptococcus neoformans and Cryptococcus gattii in the molecular mechanisms governing utilization of D-amino acids as the sole nitrogen source. PLoS One 10: e0131865, 2015. PubMed: 26132227 Janbon G, et al. Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation. PLoS Genet 10: e1004261, 2014. PubMed: 24743168 Hagen F, et al. Recognition of seven species in the Cryptococcus gattii/Cryptococcus neoformans species complex. Fungal Genet. Biol. 78: 16-48, 2015. PubMed: 25721988 Peter R Williamson, personal communication |