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| 产品名称： | pWS10 |
|---|---|
| 商品货号： | TS185833 |
| Designations： | pWS10 |
| GenBank Number： | M24114 |
| Species： | Wound tumor virus |
| Depositors： | DL Nuss |
| Vector： | Construct size (kb): 4.039999961853027 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.169999957084656 Gene product: segment S10, Pns11 polypeptide |
| Insert Size (kb)： | 1.170 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | HindIII cuts the insert twice, 7 bp apart. The insert contains internal EcoRI, AvaI, SalI, AccI, HincII, PstI and HindIII sites. Restriction digests of the clone give the following sizes (kb): BamHI--3.1, 1.2; EcoRI--3.1, 0.83, 0.41; HindIII--3.9, 0.38; PstI--3.4, 0.92; XbaI--4.3. Linearize the plasmid with SphI for in vitro transcription. This is a full-length clone of virus segment S10. The cDNA ends were tailored to terminal BamHI sites before cloning into the BamHI site of pGEM-1. |
| Classification： | Phytoreovirus |
| References： | Xu ZK, et al. Assignment of wound tumor virus nonstructural polypeptides to cognate dsRNA genome segments by in vitro expression of tailored full-length cDNA clones. Virology 168: 73-78, 1989. PubMed: 2909991 Donald L Nuss, personal communication |