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| 产品名称： | pRS327 |
|---|---|
| 商品货号： | TS185916 |
| Designations： | pRS327 |
| Depositors： | DJ Stillman |
| Biosafety Level： | 1 |
| Host： | Escherichia coli DH5α |
| Vector Information： | Size (kb): 9.56
Vector: pRS327 (plasmid)
Type: shuttle
Construction: pUC
Marker(s): AmpR, LYS2
Features: eukaryotic selectable marker cassette: LYS2 insert detection: LacZ |
| Applications： | Cloning vector |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Sikorski RS, Boeke JD. In vitro mutagenesis and plasmid shuffling: from cloned gene to mutant gene. Methods Enzymol. 194: 302-318, 1991. PubMed: 2005795 Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436 Brachmann CB, et al. Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast 14: 115-132, 1998. PubMed: 9483801 Eriksson P, et al., pRS yeast vectors with a LYS2 marker. Biotechniques 36: 212-213, 2004. |
| Shipped： | frozen |
| Shipping Information： | Frozen Escherichia coli DH5α containing the plasmid. |