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| 产品名称： | pBYr5aSP |
|---|---|
| 商品货号： | TS185920 |
| Designations： | pBYr5aSP |
| Species： | Bean yellow mosaic virus |
| Depositors： | J Hammond |
| Vector： | Construct size (kb): 3.75 |
| Insert： | DNA: cDNA Insert lengths(kb): 0.75 Gene product: genome, partial |
| Insert Size (kb)： | 0.75 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | The insert has an internal HindIII site about 180 bp from the vector EcoRI site. The insert also includes DdeI sites (2), TaqI sites (2), Sau3A1 istes (2), and a RsaI site. Restriction digests of the clone give the following sizes (kb): PstI--3.0, 0.75; EcoRI--3.8; HindIII--3.2, 0.6. Also available from the ATCC are pBYr5 (ATCC 45074) and pBYr5bSP (ATCC 45076) which contain the 0.75 kb PstI fragment. The former is cloned in pUC9 and the latter is also in pSP64, but in the opposite orientation. pBYr5aSP was constructed by random priming. RNA transcribed from the SP6 promoter is the virion sense strand, and will hybridize to (-) strand RNA. |
| Classification： | Potyvirus |
| References： | John Hammond, personal communication |