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微信小章| 产品名称： | Porcine reproductive and respiratory syndrome virus |
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| 商品货号： | TS185934 |
| Classification： | Arteriviridae, Arterivirus |
| Deposited As： | Porcine respiratory and reproductive syndrome virus |
| Agent： | Porcine reproductive and respiratory syndrome virus |
| Strain： | ISU-12 |
| Applications： | This virus produces respiratory and reproductive disease in pigs. Strain is used for the production of vaccine against porcine respiratory and reproductive syndrome. |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation： | pneumonic pig lungs, United States |
| Product Format： | frozen |
| Storage Conditions： | -70°C |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Comments： | This virus produces respiratory and reproductive disease in pigs. Strain is used for the production of vaccine against porcine respiratory and reproductive syndrome. Inoculate 2-3 day-old confluent monolayers with undiluted or 1:10 dilution of virus stock. After 1 hour add culture medium containing 2% FBS. |
| Effect on Host： | tumorigenicity: Cytopathic effects (degeneration, rounding, and clumping of cells) CPE characterized by cell rounding and degeneration, cell clumping and clustering tumorigenicity: Presence of virus can be confirmed with conjugated monoclonal antibody. |
| Growth Medium： | Dulbeccos modified Eagles medium with 5% fetal bovine serum
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| Recommended Host： | PSP-36 cells (ATCC CRL-11171) Host of Choice: PSP-36-SAH cellsxa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0xa0 Host Range: PSP-36-SAH cells and pigs PSP-36-SAH cells; pig |
| Growth Conditions： | Temperature: 37.0°C Duration: 3 days at 37C in PSP-36 cells (ATCC CRL-11171) |
| Name of Depositor： | Iowa State Univ. Res. Fndn. |
| U.S. Patent： | |
| U.S. Patent Number： | |
| Source： | pneumonic pig lungs, United States |
| References： | Paul PS, et al. Highly virulent porcine reproductive and respiratory syndrome viruses which produce lesions in pigs and vaccines that protect pigs against SAID syndrome. US Patent 5,695,766 dated Dec 9 1997 Paul PS. Porcine reproductive and respiratory syndrome. J. Clin. Vet. Med. 11: 19-28, 1993. |