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| 产品名称： | pAVE1 pAVE-1 |
|---|---|
| 商品货号： | TS185940 |
| Designations： | pAVE1 pAVE-1 |
| Depositors： | HA Barrera Saldana |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 2.7869999408721920 Vector: pAVE1 (plasmid) Promoters: Promoter hCMV Construction: pCMVcat, pUC19 Marker(s):ampR Construct size (kb): 2.786999940872192 Features: marker(s): ampR promoter: hCMV replicon: pMB1 enhancer: hCMV |
| Applications： | expression vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.1; PstI--2.3, 0.8; XbaI--2.4, 0.7. Commercial use of the human cytomegalovirus promoter/enhancer has been patented on behalf of B. Fleckenstein and W. Schaffner by the Beringwerke. Interested parties should contact the patent holders before planning a commercial application. Shuttle expression vector. Constructed by ligating the larger HindIII/EcoRI fragment of pCMVcat (removing CAT and the SV40 polyadenylation signals) to the HindIII/EcoRI multiple cloning site of pUC19. The order of the major features in this plasmid is: hCMV enhancer/promoter - HindIII - MCS - EcoRI - bla - ori. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Ramirez-Solis R, et al. New vectors for the efficient expression of mammalian genes in cultured cells. Gene 87: 291-294, 1990. PubMed: 2158929 |
| Shipped： | freeze-dried |