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微信小章| 产品名称： | Pseudallescheria angusta (Malloch et Cain) McGinnis et al. |
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| 商品货号： | TS185964 |
| Deposited As： | Scedosporium apiospermum |
| Strain Designations： | SC1 |
| Application： | Antifungal susceptibility testing Ref   Ghannoum MA, et al. Interlaboratory study of quality control isolates for a broth microdilution method (modified CLSI M38-A) for testing susceptibilities of dermatophytes to antifungals. J. Clin. Microbiol. 44: 4353-4356, 2006. RefEspinel-Ingroff A, et al. Quality control and reference guidelines for CLSI broth microdilution susceptibility method (M38-A document) for amphotericin B, itraconazole, posaconazole, and voriconazole. J. Clin. Microbiol. 43: 5243-5246, 2005. |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | no |
| Preceptrol®： | no |
| Comments： | This organism is a CLSI control strain for antimicrobial susceptibility testing. This fungus was re-identified as Pseudallescheria angusta based on the multigene DNA sequence comparison. |
| Morphology： | After 5 days cleistothecia form, immersed, membranaceous, spherical, light brown to black. Erect synnemata may be present, producing broadly clavate, subhyaline or pale brown conidia. Conidiogenous cells also arising from undifferentiated hyphae, cylindrical, producing slimy heads of 1-celled, smooth-walled, subhyaline to brown, subspherical to elongate conidia. |
| Medium： | ATCC® Medium 325: Malt extract agar (Blakeslees formula) ATCC® Medium 336: Potato dextrose agar (PDA) ATCC® Medium 200: YM agar or YM broth |
| Growth Conditions： | Temperature: 25°C to 30°C
Atmosphere: Typical aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence GGTCTCCGTTGGTGAACCAGCGGAGGGATCATTACAGAGTTACTACTCCAAACCCATTGTGAACCTTACCTATGTTCTGTTGCCTCGGCGGCGTGGTCAGCGCCCCTCTGAAAAGAGGACGATGCCCTCCCGCCGGCAGCACCAAACTCTTGAATTTTACAGCGGATTACAATTCTGATTTGAAAACAAAAAACAAGTTAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCGGCAGTAATCTGCCGGGCATGCCTGTCCGAGCGTCATTTCAACCCTCGAACCTCCGTTTCCTTAGGGAAGCTCAGGGTCGGCGTTGGGGCGCTACGGCGAGTCTTCGCGACCCCCGTAGGCCCTGAAATACAGTGGCGGTCCCGCCGCGGTTGCCTTCTGCGTAGTAAGTCTCTTTTGCAAGCTCGCATTGGGTCCCGGCGGAGGCCTGCCGTCAAACCTATAACTTCAGATGGTTTGACCTCGGATCAGGTAGGGTTACCCGCTGAACTTAAGCATATCAATAA D1D2 region of the 28S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAACAGCTCAAATTTGAAATCTGGCGGCCTCTGGGCCGTCCGAGTTGTAATTTGAAGAGGATGCTTTTGGCGAGGTGCCTTCCGAGTGCCCTGGAACGGGACGCCACAGAGGGTGAGAGCCCCGTATGGTTGGACGCCGAGCCTCTGTAAAGCTCCTTCGACGAGTCGAGTAGTTTGGGAATGCTGCTCAAAATGGGAGGTAAACCCCTTCTAAAGCTAAATACTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGAAAAGAGAGTTAAACAGCACGTGAAATTGTTGAAAGGGAAGCGCTTGCGACCAGACTTGTGCTCGTCGAATCAGCCGCCGCTCGTCGGCGGCGCACTTCGGCGGGCTCAGGCCAGCATCAGTTCGCTGCAGGGGGAGAAAGGCGATGGGAATGTGGCTCTTCGGAGTGTTATAGCCCGCCGCGCAATACCCCTCGGCGGACTGAGGACCGCGCATCTGCAAGGATGCTGGCGTAATGGTCGTCAGCGAC Beta-tubulin gene (bTub) CCCCCCTCCCATTCCGAGCTCGCGATATATCCTATCTCAGGATCCATCTCGACCTTCATCATCGTCACTCGATGCTCTTTTGCCATCGTCTGGCGGTGAAGCTGTCGTCGGGCAATATTGCGACGCGTTCAAGCTTGCCTCCTTCAACGGCGGTGCACTTGGTGACCCCGCCCACAGGACGAGACATAGCTTGAAGAGATGCAGCCTCGTCCAATAAACCATCCAATGGGTTAAGTTTCGAATGCTAACGTATATTTTCTTTTTCCTCGTGTACAGGTTCATCTGCAGACAGGCCAGTGCGTAAGTAAATTCTTGAGGACCCTATGTCCTTTTCCCCCCTGCTTCCATTTCCCTTCTGGGCCATGATCTTGTCTGCGGGGCTAACGTTTATTTTTGCCCCCCTTTCTAGGGAAACCAAATTGGTGCTGCCTTCTGGTGCGTACATTTTTCCCGACGACGCGCGATCGAGAAACGGATGCGAATATAATTCTTCTGTGCGAGATATTTCTATTTGATGGACAGAGTACTTATCGTATTTTTAGGCAAACCATTTCTGGCGAGCACGGTCTTGATAGCAACGGAGTGTACGGAACCCCCTCATCCCTTTCCCCCCCAAATGCCAGTCACATCGACTGGTGCTGCTCTCACATTCACGGCAGACACTGATTGTCCCTTATACGTCAAAGTTACAATGGCACTTCTGAACTCCAGCTTGAGCGCATGAGCGTCTACTTCAACGAGGTAAGTTTTTGGTTCAAACCATGGGCTGGTTGGTGTGGTGTCATCCGGCCTCCGTTGTTGCAGAAAATACTAACGTATTGGTTACCTCCACATTATAGGCTTCGGGCAACAAATATGTTCCTCGCGCTGTCCTTGTAGATCTCGAGCCCGGTACCATGGACGCCGTCCGT |
| Name of Depositor： | TJ Walsh |
| Special Collection： | NSF - Mycology |
| Chain of Custody： | ATCC |
| Isolation： | Human heart |
| References： | Reference Method for Broth Dilution Antifungal Susceptibility Testing of Filamentous Fungi: Approved Standard - 2nd Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M38-A2. Ghannoum MA, et al. Interlaboratory study of quality control isolates for a broth microdilution method (modified CLSI M38-A) for testing susceptibilities of dermatophytes to antifungals. J. Clin. Microbiol. 44: 4353-4356, 2006. Espinel-Ingroff A, et al. Quality control and reference guidelines for CLSI broth microdilution susceptibility method (M38-A document) for amphotericin B, itraconazole, posaconazole, and voriconazole. J. Clin. Microbiol. 43: 5243-5246, 2005. |