Construct size (kb): 3.450000047683716

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ, primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1
marker(s): ampR, Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: VCL
Gene name: vinculin
Contains complete coding sequence?: N
Chromosome: 10; Localization: 10 q22.1-q23; 10, 446.910000 cR
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 0.45
Cross references: DNA Seq. Acc.: M33308; EST: EST04831
Nucleotides 1-273 of the insert correspond to
nucleotides 4566-4294 of M33308 with 99% identity.
DNA Seq. Acc.: T06942; EST: EST04831; Primer: M13-21; THC
id: THC78924

Insert lengths(kb): 0.449999988079071

Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)

Gene product: vinculin VCL

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): EcoRI--3.1, 0.45.

Based on the alignment with GenBank accession M33308 (5102 nt, CDS 51-3251) and the size of the insert, this clone appears to contain only 3 untranslated sequence.