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| 产品名称： | pFRK1 |
|---|---|
| 商品货号： | TS186020 |
| Designations： | pFRK1 |
| Species： | Mus musculus, mouse |
| Depositors： | R Klein, M Barbacid |
| Vector： | Construct size (kb): 6.099999904632568 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.171000003814697 Tissue: brain Gene product: trkB, tyrosine kinase receptor(tyrosine kinase trkB) Trkb |
| Insert Size (kb)： | 3.171 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The following enzymes cut the insert once (in nucleotides from the 5 end of the insert): XhoI--209; PvuII--372; SalI--481; HindIII--2410; PstI--2493; BamHI--2774. Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 2.9; HindIII--5.3, 0.78; PstI--3.9, 2.1; XbaI--6.1; HincII--3.4, 1.4, 1.3. This clone encodes a portion of the extracellular region and the complete transmembrane and tyrosine kinase catalytic domains. It includes a 1376 bp 3 non-coding region. There are no consensus sequences for polyadenylation near the 3 terminus, but there are 4 ATTT(A) motifs which have previously been identified in lymphokine and oncogene mRNA and which have been associated with message instability. This probe detects genomic BamHI fragments of 4.6, 3.3, and 1.5 kb in humans. The probe detects diverse mRNA species which are tissue-specific and developmentally regulated. Sizes are given in kb. Brain: 9.0, 8.2, 8.0, 5.5, 3.5, 2.5, 2.0; lung and muscle: 8.2, 8.0, 3.5, 2.5, 2.0; ovary: 8.2, 2.5; testis: 2.8, 2.5. |
| Classification： | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References： | Klein R, et al. trkB, a novel tyrosine protein kinse receptor expressed during mouse neural development. EMBO J. 8: 3701-3709, 1989. PubMed: 2555172 Klein R, et al. The trk proto-oncogene encodes a receptor for nerve growth factor. Cell 65: 189-197, 1991. PubMed: 1849459 Rudiger Klein, personal communication |