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微信小章| 产品名称： | H5.46 546 |
|---|---|
| 商品货号： | TS186156 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Its neutralizing activity confers protection in C57BL/6J mice (sIgM-/-) that were deficient in the production of secreted IgM but capable of expressing surface IgM PubMed: 14662909. The antibody reacts with a virus specific epitope, the E protein. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against West Nile Virus |
| Cellular Products： | immunoglobulin; monoclonal antibody; against West Nile Virus |
| Comments： | The antibody reacts with a virus specific epitope, the E protein. Its neutralizing activity confers protection in C57BL/6J mice (sIgM-/-) that were deficient in the production of secreted IgM but capable of expressing surface IgM PubMed: 14662909. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with:
10% NCTC 109
1% OPI (0.15 mg/ml oxaloacetate, 0.05 mg/ml pyruvate, 0.0082 mg/ml bovine insulin)
15% fetal bovine serum
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL.xa0 Maintain cultures at a cell concentration between 1 x 105 and 2 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL. Medium Renewal:xa0Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | mouse IgM kappa |
| Name of Depositor： | RB Tesh |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Gould EA, et al. Antigencity of flaviviruses. Arch. Virol. Suppl. 1: 137-152, 1990. The antibody reacts with a virus specific epitope. Diamond MS, et al. A critical role for induced IgM in the protection against West Nile virus infection. J. Exp. Med. 198: 1853-1862, 2003. PubMed: 14662909 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |