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| 产品名称： | pLD25 FD 27533, FD 27534, FD 27553 carrying plasmid pLD25 |
|---|---|
| 商品货号： | TS186162 |
| Designations： | pLD25 FD 27533, FD 27534, FD 27553 carrying plasmid pLD25 |
| Depositors： | Pfizer, Inc. |
| U.S. Patent： | |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 11.0000000000000000 Vector: pLD25 (plasmid) Type: YI-type (integrating) shuttle vector Construction: pBR322, Y. lipolytica NRRL Y-1094 Marker(s):LEU2,ampR Construct size (kb): 11.0 Features: marker(s): ampR, LEU2 replicon: pMB1 |
| Applications： | YI-type (integrating) shuttle vector contains sequence 3-isopropylmalate dehydrogenase beta-isopropylmalate dehydrogenase shuttle vector |
| Comments： | The Y. lipolytica portion of the plasmid contains the following restriction sites: EcoRI (5); SphI (1); SalI (2); AvaI (8); KpnI (1); EcoRI (4); XhoI (2), BglII (2). It lacks HindIII, ClaI, BamHI, and NruI sites. Replicates autonomously in E. coli and integrates but does not replicate autonomously in Y. lipolytica. Constructed by a inserting a 6.6 kb Sau3AI fragment (partial) containing LEU2 into the BamHI site of pBR322. The gene is contained in an EcoRI fragment (partial) of approximately 2.4 kb. |
| Media： | ATCC® Medium 1245: YEPD |
| Growth Conditions： | Temperature: 25.0°C |
| References： | Davidow LS, DeZeeuw JR. Process for transformation of Yarrowia lipolytica. US Patent 4,880,741 dated Nov 14 1989 |
| Shipped： | freeze-dried |