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| 产品名称： | YEp436 pRB697 |
|---|---|
| 商品货号： | TS186188 |
| Designations： | YEp436 pRB697 |
| Depositors： | D Botstein |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 13.3000001907348600 Vector: YEp436 (plasmid) Construction: pSI4, YIp5 Marker(s):URA3,ampR,LEU2-d Construct size (kb): 13.30000019073486 Features: marker(s): ampR, LEU2-d, URA3 replicon: pMB1, 2 micron |
| Applications： | YE-type (episomal) shuttle vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI/XbaI--6.0, 4.5, 2.8; SalI--13.5; SmaI--13.5. LEU2-d is a promoter-defective allele of the yeast LEU2 gene, which increases plasmid copy number to 200-300 copies in the presence of LEU2 selection (host grown in medium lacking leucine). YE-type shuttle vector. The order of the major features in this plasmid is: LEU2-d - ori(2 micron) - BamHI site - SalI site - URA3 - ampR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Ma H, et al. Plasmid construction by homologous recombination in yeast. Gene 58: 201-216, 1987. PubMed: 2828185 Rose AB, Broach JR. Propagation and expression of cloned genes in yeast: 2-micron circle-based vectors. Methods Enzymol. 185: 234-279, 1990. PubMed: 2199781 |
| Shipped： | freeze-dried |