微信小陈
微信小章| 产品名称： | P18 p18 |
|---|---|
| 商品货号： | TS186214 |
| Designations： | P18 p18 |
| GenBank Number： | J04211 |
| Species： | Homo sapiens, human |
| Depositors： | M Chu |
| Applications： | This probe encodes an epitope of collagen type VI since it was identified by immunoscreening a lambda gt11 library. |
| Vector： | Construct size (kb): 4.699999809265137 |
| Insert： | DNA: cDNA Insert lengths(kb): 2.0 Tissue: placenta Gene product: collagen, type VI, alpha 1 COL6A1 Alleles: b, s, , A2, B1, B2, C1, C2, C3, A1 |
| Insert Size (kb)： | 2.0 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert contains 1 KpnI site (in the region encoding the triple-helical domain) and 5 PstI sites. Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 2.0; HindIII--4.7; BamHI/EcoRI--2.7, 2.0; PstI--3.2, 0.8, 0.5, 0.2; XbaI--4.7. COL6A1 and COL6A2 are closely linked (about 200 kb). This probe encodes an epitope of collagen type VI since it was identified by immunoscreening a lambda gt11 library. The insert encompasses that portion of the cDNA from about 1.2 kb to 3.2 kb from the 5 end, including the 3 portion of the triple-helical domain. The probe detects the following genomic TaqI fragments (kb): human specific--6.0, 4.4 and/or 4.1 (polymorphic); mouse--6.6; Chinese hamster--2.0. This probe detects a mRNA of 4.2 kb in human fibroblasts. |
| References： | Chu ML, et al. Sequence analysis of alpha1(VI) and alpha2(VI) chains of human type VI collagen reveals internal triplication of globular domains similar to the A domains of von Willebrand facter and two alpha2(VI). EMBO J. 8: 1939-1946, 1989. PubMed: 2551668 Chu ML, et al. Amino acid sequence of the triple-helical domain of human collagen type VI. J. Biol. Chem. 263: 18601-18606, 1988. PubMed: 3198591 Francomano CA, et al. The COL6A1 and COL6A2 genes exist as a gene cluster and detects highly informative DNA polymorphisms in the telomeric region of human chromosome 21q. Hum. Genet. 87: 162-166, 1991. PubMed: 1676701 Justice MJ, et al. A genetic linkage map of mouse chromosome 10: localization of eighteen molecular markers using a single interspecific backcross. Genetics 125: 855-866, 1990. PubMed: 1975791 Weil D, et al. Cloning and chromosomal localization of human genes encoding the three chains of type VI collagen. Am. J. Hum. Genet. 42: 435-445, 1988. PubMed: 3348212 |