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| 产品名称： | pVT64 |
|---|---|
| 商品货号： | TS186225 |
| Designations： | pVT64 |
| Depositors： | VN Trieu |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli JM109 |
| Vector Information： | Size (kb): 6.8000001907348630 DESCRIPTION OF VECTOR: Intact vector size: 6.800 Type of vector: plasmid Cloning sites: PstI HincII ScaI SspI Polylinker sites: Construction: pDM2, pBGS8 Host range: Haemophilus influenzae; Escherichia coli Features (with orientation and position when available): marker(s): ampR, spcR replicon: pMB1, pRSF0885 Vector: pVT64 (plasmid) Construction: pDM2, pBGS8 Marker(s):ampR,spcR Construct size (kb): 6.800000190734863 Features: marker(s): ampR, spcR replicon: pMB1, pRSF0885 |
| Applications： | shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): PstI--6.8; ScaI--6.8; SspI--6.8; BamHI--4.2, 2.4. Cloning into the HincII, PstI, ScaI, or SspI sites inactivates ampR. Precautions have not been taken to maintain the F in the JM109 host. The F is not required to use this plasmid. Shuttle vector. The order of the major features in this plasmid is: pMB1 ori - ampR - pRSF0885 rep - spcR. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Trieu VN, McCarthy D. Improved plasmid shuttle vectors for Haemophilus influenzae and Escherichia coli. Gene 86: 99-102, 1990. PubMed: 2179060 Vuong N Trieu, personal communication |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |