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| 产品名称： | M13K07 |
|---|---|
| 商品货号： | TS186261 |
| Designations： | M13K07 |
| Depositors： | P Dwivedi |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 8.6999998092651370 Vector: M13K07 (phage, lambda - replacement) Construction: M13, gene II of M13mp1, pM15A ori, Tn903 Marker(s):kanR Construct size (kb): 8.699999809265137 Features: marker(s): kanR replicon: M13 |
| Applications： | vector permitting production of single-stranded DNA |
| Comments： | Restriction digests analyzed on agarose gels give the following sizes (kb): BamHI--8.7; HindIII--8.7. There is some instability of the insert during growth if not propagated correctly. This a helper bacteriophage for production of single-stranded DNA from plasmids containing M13 intergenic region which preferentially packages single-stranded plasmid DNA over phage DNA. |
| References： | Vieira J, Messing J. Production of single-stranded plasmid DNA. Methods Enzymol. 153: 3-11, 1987. PubMed: 3323803 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried bacteria-free phage |