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| 产品名称： | Dengue virus type 2 |
|---|---|
| 商品货号： | TS186275 |
| Classification： | Flaviviridae, Flavivirus, Dengue virus |
| Agent： | Dengue virus type 2 |
| Strain： | TH-36 |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1.0 mL |
| Storage Conditions： | -70°C or colder |
| Images： |  |
| Comments： | ATCC® VR-1810™ was adapted to growth in ATCC® CRL-1660™ by serial passaging in vivo product, ATCC® VR-345™. ATCC® VR-345™ and ATCC® VR-1810™ have not been compared for sequence similarity or infectivity. |
| Effect on Host： | Little to no CPE expected. Detect by immunofluorescence or molecular based procedures.
Syncytial formation may occur in high MOI infections. |
| Recommended Host： | Aedes albopictus clone C6/36, Whole Larva Asian Tiger Mosquito (ATCC® CRL-1660™) |
| Growth Conditions： | Temperature: 28°C Recommendations for Infection: For best results, infection should be performed on a 60-80% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 28°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 5-7 days at 28°C in a humidified 5% CO2 atmosphere. |
| Name of Depositor： | Dr. William Hammon, Graduate School of Public Health, University of Pittsburgh |
| Chain of Custody： | ATCC |
| Source： | Serum from patient diagnosed as Thai hemorrhagic fever, Thailand, 1958 |
| References： | Hammon WM, et al. Viruses associated with epidemic hemorrhagic fevers of the Philippines and Thailand. Science 131(3407): 1102-1103, 1960. PubMed: 14399343 Hammon WM et al. Virological findings in the 1960 hemorrhagic fever epidemic (dengue) in Thailand. Am. J. Trop. Med. Hyg. 13: 629-641, 1964. PubMed: 14196063 |