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| 产品名称： | c9666 |
|---|---|
| 商品货号： | TS186363 |
| Designations： | c9666 |
| GenBank Number： | U10397 U10398 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | L Riles, MV Olson |
| Vector： | Construct size (kb): 44.40000152587891 |
| Insert： | DNA: genomic Insert lengths(kb): 37.94900131225586 Gene product: recombination protein, meiotic DCD1 |
| Insert Size (kb)： | 37.949 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--7.5, 4.2, 3.7 and smaller bands. Reported to contain EcoRI/HindIII fragments of the following sizes (kb): 7.421, 0.401 (vector); 4.193, 3.761, 3.064, 2.750, 2.416, 2.210, 2.104, 1.738, 1.738, 1.676, 1.276, 1.139, 0.961, 0.879, 0.824, 0.821, 0.694, 0.694, 0.642, 0.499, 0.469, 0.394. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Corresponds to nt 382002-419951 of the sequence of chromosome VIII. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Gimeno CJ, Fink GR. Induction of pseudohyphal growth by over expression of PHD1, a Saccharomyces cerevisiae gene related to transcriptional regulators of fungal development. Mol. Cell. Biol. 14: 2100-2112, 1994. PubMed: 8114741 Linda Riles, personal communication |