微信小陈
微信小章| 产品名称： | WT SV40 MEF |
|---|---|
| 商品货号： | TS186420 |
| Organism： | Mus musculus, mouse |
| Tissue： | embryonic fibroblast |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast-like |
| Culture Properties： | adherent |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | This cell line can be paired with MEF cell lines, generated from BCL-2 family member knockout mice (e.g., ATCC CRL-2908, ATCC CRL-2909, ATCC CRL-2910, ATCC CRL-2911, ATCC CRL-2912, ATCC CRL-2913),xa0as a control to study the molecular mechnaisms of cell apoptosis and thexa0BCL-2 family signaling pathway.
|
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | These cells are SV40 immortalized mouse embryonic fibroblasts (MEFs), which were generated from wild type mice. |
| Tumorigenic： | no |
| Comments： | These cells are SV40 immortalized mouse embryonic fibroblasts (MEFs), which were generated from wild type mice. This cell line can be paired with MEF cell lines, generated from BCL-2 family member knockout mice (e.g., ATCC CRL-2908, ATCC CRL-2909, ATCC CRL-2910, ATCC CRL-2911, ATCC CRL-2912, ATCC CRL-2913),xa0as a control to study the molecular mechnaisms of cell apoptosis and thexa0BCL-2 family signaling pathway. The cells will slough off of the growth surface if they become too heavy. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated IMDM Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: 10 % Fetal Bovine Serum and 1x Non-essential amino acids.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation ratio: A subcultivation ratio of 1:8 to 1:20 is recommended. Medium renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: 90% Fetal Bovine Serum and 10% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor： | S Korsmeyer |
| References： | Wei M, et al. Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death. Science 292(5517): 727-730, 2001 PubMed: 11326099 Lindsten T, et al. The Combined Functions of Proapoptotic Bcl-2 Family Members Bak and Bax Are Essential for Normal Development of Multiple Tissues. Molecular Cell 6(6): 1389-1399, 2000 PubMed: 11163212 Zong WX, et al. BH3-only proteins that bind pro-survival Bcl-2 family members fail to induce apoptosis in the absence of Bax and Bak. Genes Dev. 15(12): 1481-6, 2001 PubMed: 11410528 Cheng EH, et al. BCL-2, BCL-XL Sequester BH3 Domain-Only Molecules Preventing BAX- and BAK-Mediated Mitochondrial Apoptosis. Molecular Cell 8(3) : 705–711, 2001 PubMed: 11583631 |