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微信小章| 产品名称： | Human rhinovirus 2 |
|---|---|
| 商品货号： | TS186524 |
| Classification： | Picornaviridae, Enterovirus, Rhinovirus A |
| Deposited As： | Rhinovirus 2 |
| Agent： | Human rhinovirus 2 |
| Strain： | HGP |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Comments： | Properties similar to other viruses in the Picornaviridae family, except unstable at pH 3-5. No hemagglutinins demonstrated. Reported as non-pathogenic for common laboratory animals. |
| Effect on Host： | CPE, sloughing, rounding and clumping |
| Recommended Host： | H1-HeLa (ATCCxa0CRL-1958) |
| Growth Conditions： | Temperature: 33°C Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 85-95% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 33°C in a humidified 5% CO2 atmosphere while rocking continuously. End adsorption by adding virus growth medium. Incubation: 1-6 days while rocking continuously |
| Name of Depositor： | RM Conant, VV Hamparian, The Ohio State University |
| U.S. Patent： | |
| U.S. Patent Number： | |
| Source： | Nasal washing from patient with cold |
| References： | Taylor-Robinson D, Tyrrell DA. Serotypes of viruses (Rhinoviruses) isolated from common colds. Lancet 1: 452-454, 1962. PubMed: 13920011 Tyrrell DA, et al. Some virus isolations from common colds. I. Experiments employing human volunteers. Lancet. 1(7118): 235-237, 1960. PubMed: 13840112 Tyrrell DA, Bynoe ML. Some further virus isolations from common colds. Br. Med. J. 1(5223): 393-397, 1961. PubMed: 13778900 |