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| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS186531 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | RR1(pRK248) |
| Application： | transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen |
| Preceptrol®： | no |
| Genotype： | F- supE44 lacY1 ara-14 galK2 xyl-5 mtl-1 leuB6 proA2 hsdS20 rpsL20 lambda- (pRK248) |
| Type Strain： | no |
| Comments： | The depositor recommends picking a single colony from a plate containing tetracycline to grow for stock. A recombination-proficient host for use with lambdaZd vectors (ATCC 37563-37565) and for releasing plasmid inserts from lambdaZd libraries and clones. Incoming lambdaZd phage are maintained as large plasmids at 32C. a host containing a temperature sensitive lambda repressor gene (cI857) on a ColE1-compatible plasmid |
| Medium： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Growth Conditions： | Temperature: 30.0°C |
| Name of Depositor： | AJ Murphy |
| References： | Bernard HU, Helinski DR. Use of the lambda phage promoter PL to promote gene expression in hybrid plasmid cloning vehicles. Methods Enzymol. 68: 482-492, 1979. PubMed: 232223 Murphy AJ, Efstratiadis A. Cloning vectors for expression of cDNA libraries in mammalian cells. Proc. Natl. Acad. Sci. USA 84: 8277-8281, 1987. PubMed: 3479791 Andrew J Murphy, personal communication |