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| 产品名称： | pSZ62 |
|---|---|
| 商品货号： | TS186697 |
| Designations： | pSZ62 |
| Depositors： | JW Szostak |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.0999999046325680 Vector: pSZ62 (plasmid) Promoters: Promoter none Construction: pBR322 Marker(s):ampR,HIS3 Construct size (kb): 6.099999904632568 Features: marker(s): ampR, HIS3 promoter: none replicon: pMB1 terminator: none enhancer: none |
| Applications： | YI-type (integrating) shuttle vector integrating vector shuttle vector |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--6.1; BamHI--4.4, 1.7; BglII--5.4, 0.7; SalI/XhoI--4.6, 1.5; EcoRI/SalI--3.7, 2.4; SalI--6.1. Integration YI-type shuttle vector. Constructed by inserting a 1.7 kb HIS3-containing BamHI fragment into the BamHI site of pBR322. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Orr-Weaver TL, et al. Yeast transformation: a model system for the study of recombination. Proc. Natl. Acad. Sci. USA 78: 6354-6358, 1981. PubMed: 6273866 |
| Related Products： | component of:ATCC 77188 |
| Shipped： | freeze-dried |