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| 产品名称： | pBRH3B |
|---|---|
| 商品货号： | TS186786 |
| Designations： | pBRH3B |
| Depositors： | RL Rodriguez |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 7.6999998092651370 Vector: pBRH3B (plasmid) Promoters: Promoter none Construction: pBR316 Marker(s):ampR Construct size (kb): 7.699999809265137 Features: insert detection: tetR marker(s): ampR promoter: none replicon: pMB1 |
| Applications： | promoter-cloning vector |
| Comments： | This is a promoter cloning plasmid vector using expression of tetracycline resistance for selection. This was constructed by inserting an oligonucleotide containing an EcoRI site into the HindIII site of pBR316, inactivating the tet promoter. It is resistant to low levels of tetracycline without an insert and can be used to clone only strong promoters. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | West RW Jr., et al. Construction and characterization of E. coli promoter-probe plasmid vectors. I. Cloning of promoter-containing DNA fragments. Gene 7: 271-288, 1979. PubMed: 118083 |
| Shipped： | freeze-dried |