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| 产品名称： | Escherichia coli (Migula) Castellani and Chalmers |
|---|---|
| 商品货号： | TS186834 |
| Classification： | Enterobacteriaceae, Escherichia |
| Strain Designations： | DK22 |
| Application： | in vitro packaging (bacteriophage lambda/cosmid) transformation host |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Preceptrol®： | no |
| Genotype： | F- delta(srl-recA)306 araD139 delta(ara-leu)7697 delta(lacIPOZY)X74 galU galK hsdR rpsL mcrA mcrB lambda 3169(b2red3imm434cItsSam7) |
| Type Strain： | no |
| Comments： | After in vitro packaging and transfection, cosmid libraries may be established by antibiotic selection at 32C. Libraries may be subsequently packaged directly in vivo by shifting to 42C. A lambda lysogen of DK1 (ATCC 35691) After in vitro packaging and transfection, cosmid libraries may be established by antibiotic selection at 32C. Libraries may be subsequently packaged directly in vivo by shifting to 42C. This recA- host strain is a useful in vivo packaging host for cosmids that utilize lambda cohesive ends. |
| Medium： | ATCC® Medium 1065: LB Agar/Broth, Miller |
| Growth Conditions： | Temperature: 30.0°C |
| Name of Depositor： | DM Kurnit |
| References： | Kurnit DM. Escherichia coli recA deletion strains that are highly competent for transformation and for in vivo phage packaging. Gene 82: 313-315, 1989. PubMed: 2684784 |