| 产品名称: | pACHRAcDNA ACh receptor alpha cDNA |
|---|---|
| 商品货号: | TS186941 |
| Designations: | pACHRAcDNA ACh receptor alpha cDNA |
| GenBank Number: | X03986 |
| Species: | Mus musculus, mouse |
| Depositors: | JP Merlie |
| Vector: | Construct size (kb): 4.630000114440918 |
| Insert: | DNA: cDNA Insert lengths(kb): 1.759999990463257 Tissue: muscle cell line BC3H-1 Gene product: acetylcholine receptor alpha Acra |
| Insert Size (kb): | 1.760 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried |
| Comments: | The insert contains the following restriction sites (nt position according to the published sequence): PvuII--176; BglII--319; BalI--537; BstXI--1283. Restriction digests of the clone give the following sizes (kb): EcoRI--2.8, 1.8; HindIII--4.65; BamHI--4.65; PstI--4.1, 0.5; BglII--4.65. Contains the complete coding sequence (nt 9-1382). Detects a mRNA of 2.0 kb. Oriented such that transcription from the SP6 promoter gives the sense strand. |
| Classification: | Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References: | Galili G, et al. Nucleotide sequence of the mouse muscle nicotinic acetylcholine receptor alpha subunit. Nucleic Acids Res. 14: 1511-1524, 1986. PubMed: 3456558 John P Merlie, personal communication |