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| 产品名称： | WMVII-3.2 pWMVII-3.2 |
|---|---|
| 商品货号： | TS186995 |
| Designations： | WMVII-3.2 pWMVII-3.2 |
| GenBank Number： | D00592 |
| Species： | Watermelon mosaic 2 virus |
| Depositors： | JL Slightom |
| Vector： | Construct size (kb): 5.900000095367432 |
| Insert： | DNA: cDNA Insert lengths(kb): 3.200000047683716 Gene product: nuclear inclusion protein |
| Insert Size (kb)： | 3.200 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--3.8, 2.4; EcoRI--2.7, 2.4, 1.0; PvuII--3.8, 2.4; XhoI--6.2. The insert contains the following restriction sites (nt from the 5 end): NsiI--925, 3024; EcoRI--971; BamHI--1016, HindIII--1883; XhoI--2496. Contains part of the coding sequences for the protease (to nt 658), and the complete coding sequences for the nuclear inclusion protein (nt 659-2209) and the coat protein (nt 2210-3052). |
| Classification： | Potyvirus |
| References： | Quemada H, et al. Watermelon mosaic virus II and zucchini yellow mosaic virus: cloning of 3 terminal regions, nucleotide sequences, and phylogenetic comparisons. J. Gen. Virol. 71: 1451-1460, 1990. PubMed: 2374006 |