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购买须知
| 产品名称： | pE2.8 |
|---|---|
| 商品货号： | TS186997 |
| Designations： | pE2.8 |
| Species： | Homo sapiens, human |
| Depositors： | JA Byrne, PJ Smith |
| Vector： | Construct size (kb): 5.800000190734863 |
| Insert： | DNA: genomic Insert lengths(kb): 2.799999952316284 Gene product: ribonucleotide reductase M1 polypeptide(ribonucleoside diphosphate reductase, M1 subunit) RRM1 Alleles: A2, A2, A1 |
| Insert Size (kb)： | 2.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | The insert contains a KpnI site 0.1 kb from the end of the insert closer to the XhoI site of the vector multiple cloning site. The insert does not contain restriction sites for the following enzymes: ApaI, AatII, BglII, HindIII, PvuII, SacI, XhoI. Restriction digests of the clone give the following sizes (kb): EcoRI--2.9 (doublet); KpnI--3.1, 2.7; PstI--uncut; BglI--2.4, 2.1, 1.3; SalI--uncut. The 1.8 kb EcoRI fragment (pE1.8, ATCC 65264/65265) is immediately adjacent to and downstream from a 2.8 kb EcoRI fragment (pE2.8, TS186997/65261). Contains repeated sequences which must be saturated with sheared total human placental DNA before filter hybridization to remove background. |
| References： | Bryne J, Smith P. SacI polymorphism in the RRM1 gene. Nucleic Acids Res. 18: 6177, 1990. PubMed: 1978294 Jennifer A Byrne, personal communication |