| 产品名称: | c9177 9177 |
|---|---|
| 商品货号: | TS187008 |
| Designations: | c9177 9177 |
| GenBank Number: | U00029 U00030 |
| Species: | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors: | MV Olson, L Riles |
| Vector: | Construct size (kb): 45.04000091552734 |
| Insert: | DNA: genomic Insert lengths(kb): 38.60900115966797 Gene product: protein kinase, cyclic AMP-dependent SCH9 |
| Insert Size (kb): | 38.609 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Related Products: | component of:ATCC 77284 |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments: | Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 1.517, 6.536, 0.538, 1.030, 1.374, 0.507, 2.434, 0.534, 6.513, 0.985, 0.730, 3.221, 0.658, 2.896, 3.649, 4.031. Confirmed to contain EcoRI/HindIII fragments of the following sizes (kb): 0.51, 0.65, 0.77, 0.99, 1.1, 1.4, 1.6, 2.5, 3.1, 3.4, 3.8, 4.1, 6.8. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Overlaps clones with ATCC number(s): 70098, 70403, 70487, 70675, 70767, 70890, 70899, 70923. Corresponds to nt 506978-545587 of the sequence of chromosome VIII. |
| Classification: | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References: | Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151 Johnston M, et al. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. Science 265: 2077-2082, 1994. PubMed: 8091229 Linda Riles, personal communication |