Construct size (kb): 5.400000095367432

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, insert detection: lacZ, primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, promoter: lac, replicon: pMB1, marker(s): ampR, Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PM5*
Gene name: pM5 protein
Contains complete coding sequence?: N
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 2.1
Cross references: DNA Seq. Acc.: T08008; EST: EST05899; Primer: M13-21; THC
id: THC122871
DNA Seq. Acc.: T08009; EST: EST05900; Primer: M13 Reverse;
THC id: THC122871
DNA Seq. Acc.: X57398; EST: EST05899
Nucleotides 1-221 of the insert correspond to
nucleotides 4068-3848 of X57398 with 91% identity.
DNA Seq. Acc.: X57398; EST: EST05900; Primer: M13 Reverse
Nucleotides 8-336 of the insert correspond to
nucleotides 2058-2385 of X57398 with 97% identity.

Insert lengths(kb): 2.099999904632568

Tissue: infant brain, 3 months old (from Bento Soares)

Gene product: pM5 protein PM5*

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Restriction digests of the clone give the following sizes (kb): EcoRI--4.0, 1.5; HindIII/NotI--3.4, 1.0, 0.6, 0.54.

Based on the alignment with GenBank X57398 (4086 nt, CDS 1-3573) and the size of the insert, this appears to include part of the coding sequence and the 3 untranslated region.