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| 产品名称： | pTS33 TS-33 |
|---|---|
| 商品货号： | TS187134 |
| Designations： | pTS33 TS-33 |
| GenBank Number： | M25631 |
| Species： | Homo sapiens, human |
| Vector： | Construct size (kb): 4.300000190734863 |
| Insert： | DNA: cDNA Insert lengths(kb): 1.289999961853027 Tissue: umbilical vein endothelium Gene product: thrombospondin 1 THBS1 |
| Insert Size (kb)： | 1.290 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments： | The insert contains the following restriction sites (in bp from the 5 end of the insert): BamHI--70 (5 untranslated region); NarI--234; SmaI--400; PvuII--597; PstI--737, 983. Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.25; BamHI--4.3; SmaI--3.8, 0.45; PvuII--3.6, 0.65; PstI--3.5, 0.75. The insert contains 99 nucleotides of 5 untranslated sequence and coding regions for a signal peptide of 18 amino acids and the first 379 amino acids of the mature protein. The insert detects a mRNA of 6.0 kb from rat smooth muscle cells. |
| References： | Jaffe E, et al. Mapping of the thrombospondin gene to human chromosome 15 and mouse chromosome 2 by in situ hybridization. Genomics 7: 123-126, 1990. PubMed: 2335352 Kobayashi S, et al. Partial amino acid sequence of human thromobspondin as determined by analysis of cDNA clones: homology to malarial circumsporozoite proteins. Biochemistry 25: 8418-8425, 1986. PubMed: 3030396 |