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| 产品名称： | Sp2 |
|---|---|
| 商品货号： | TS187158 |
| Designations： | Sp2 |
| GenBank Number： | M97190 |
| Species： | Homo sapiens, human |
| Depositors： | A Winoto |
| Applications： | contains sequence Sp2 transcription factor Trans-acting transcription factor 2 |
| Vector： | Construct size (kb): 5.699999809265137 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript II KS- Intact vector size: 2.961 Type of vector: phagemid Vector end: NotI Vector end: NotI Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII EcoRI PstI SmaI BamHI XbaI NotI EagI SacI Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, ->, 3-459 primer site: M13-20, -> promoter for in vitro transcription: T7, -> insert detection: lacZ, MCS: SacI...KpnI, ->, 657-759 promoter for in vitro transcription: T3, primer site: reverse, replicon: pMB1, 1032-1972 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: SP2 Gene name: Sp2 transcription factor Contains complete coding sequence?: Y Tissue: Molt13 cell line Type of DNA: cDNA Insert end: NotI Insert end: NotI linker Insert size (kb): 2.7 Cross references: DNA Seq. Acc.: M97190 Nucleotides 1-2063 of the insert correspond to nucleotides 1-2063 of M97190. Insert lengths(kb): 2.700000047683716 Tissue: Molt13 cell line Gene product: Sp2 transcription factor(Trans-acting transcription factor 2) SP2 Target Gene: Sp2 transcription factor |
| Insert Size (kb)： | 2.700 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): BglI--2.75, 1.25, 1.05, 1.0; NotI--3.0 (doublet); PstI--4.8, 1.15; PvuII--2.6, 1.8, 0.76, 0.66 +smaller fragments; SphI--6.0. Insert contains the following restriction sites (approximate kb from the 5 end): BglI--1.29; PstI--0.07, 1.14; PvuII--0.34, 0.90, 1.05, 1.14, 1.22, 1.30, 1.39, 1.46; SacI--1.72, 2.06; SmaI--1.50; SphI--1.73. |
| References： | Kingsley C, Winoto A. Cloning of GT box-binding proteins: a novel Sp1 multigene family regulating T-cell receptor gene expression. Mol. Cell. Biol. 12: 4251-4261, 1992. PubMed: 1341900 |