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| 产品名称： | pJJ283 pJJ252 |
|---|---|
| 商品货号： | TS187238 |
| Designations： | pJJ283 pJJ252 |
| Species： | Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors： | L Prakash |
| Vector： | Construct size (kb): 4.688000202178955 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC18 Intact vector size: 2.686 Type of vector: plasmid Vector end: XbaI Vector end: XbaI Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Construction: pUC71K Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ, MCS: HindIII...EcoRI, -> promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: DNA Seq. Acc.: L08752 |
| Insert： | DNA: genomic DESCRIPTION OF INSERT COMPONENT: Genome: Saccharomyces cerevisiaeCandida robusta Gene symbol: LEU2 Gene name: 3-isopropylmalate dehydrogenase Contains complete coding sequence?: U Chromosome: III; Localization: III L, 5 cM from CEN Type of DNA: genomic Insert 5 end: SalI Insert 3 end: HpaI Insert size (kb): 1.9 Cross references: Insert lengths(kb): 1.899999976158142 Gene product: 3-isopropylmalate dehydrogenase(beta-isopropylmalate dehydrogenase) LEU2 |
| Insert Size (kb)： | 1.900 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): HindIII/BamHI--2.7, 1.9; PvuII--2.3 (doublet); EcoRI--3.65, 0.95. A plasmid providing the gene for a yeast selectable marker in the MCS of pUC18, allowing more flexibility in designing constructions. Constructed by filling in the ends of both the vector and the insert fragment. LEU2 is in an orientation opposite to lacZ. The insert was subcloned from YEp13. |
| Classification： | Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References： | Jones JS, Prakash L. Yeast Saccharomyces cerevisiae selectable markers in pUC18 polylinkers. Yeast 6: 363-366, 1990. PubMed: 2220072 Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151 Louise Prakash, personal communication |