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| 产品名称： | YEpFAT7 |
|---|---|
| 商品货号： | TS187370 |
| Designations： | YEpFAT7 |
| Depositors： | K Runge |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli MC1066 |
| Vector Information： | Size (kb): 10.3179998397827200 DESCRIPTION OF VECTOR: Intact vector size: 10.318 Type of vector: plasmid Cloning sites: SacI SmaI SalI Polylinker sites: EcoRI SacI AvaI KpnI SmaI BamHI XbaI SalI PstI HindIII Other unique sites: NotI Host range: Schizosaccharomyces pombe; Saccharomyces cerevisiae; Escherichia coli Features (with orientation and position when available): promoter for in vitro transcription: T7, ->, 9016-9035 MCS: EcoRI...HindIII, ->, 10269-2 marker(s): GART, replicon: 2 micron/LEU2-d, 2086-4780 marker(s): LEU2-d, ->, 3443-4537 marker(s): URA3, promoter for in vitro transcription: T3, promoter: lac, replicon: pMB1, 6338-6338 marker(s): ampR, Vector: YEpFAT7 (plasmid) Promoters: Promoter for in vitro transcription T7 Marker(s):URA3,ampR,LEU2-d Construct size (kb): 10.31799983978272 Features: marker(s): GART marker(s): LEU2-d marker(s): URA3 marker(s): ampR promoter: lac promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: 2 micron/LEU2-d replicon: pMB1 MCS: EcoRI...HindIII |
| Applications： | Selection for URA3 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200. |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--9.6, 1.0; SmaI--11.0; HindIII--6.2, 4.8. Selection for URA3 expression produces a plasmid copy number of 20-40; growth on YC minus leucine produces a copy number of 100-200. One of 9 YE-type shuttle vectors (ATCC 87541, 87554, 87560 an d 87640 - 87645) that have the leu2-d gene, which is correlated with high copy number in S. cerevisiae, when grown in medium lacking leucine. |
| Media： | M9 + tryptophan + leucine
|
| Growth Conditions： | Temperature: 37.0°C |
| References： | Runge KW, Zakian VA. Introduction of extra telomeric DNA sequences into Saccharomyces cerevisiae results in telomere elongation. Mol. Cell. Biol. 9: 1488-1497, 1989. PubMed: 2657397 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |