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| 产品名称： | pCK92 hMCT1 |
|---|---|
| 商品货号： | TS187421 |
| Designations： | pCK92 hMCT1 |
| GenBank Number： | L31801 |
| Species： | Homo sapiens, human |
| Depositors： | JL Goldstein |
| Vector： | Construct size (kb): 5.5 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pBluescript SK- Intact vector size: 2.960 Type of vector: phagemid Vector end: SalI Vector end: PstI Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI (Eco0109I DraII ApaI) KpnI Construction: pUC19 Host range: Escherichia coli Features (with orientation and position when available): replicon: f1, -> insert detection: lacZ, primer site: M13-20, -> promoter: T7, -> MCS: KpnI...SacI, -> promoter: T3, promoter: lac, primer site: M13 reverse, replicon: pMB1 marker(s): ampR, Cross references: |
| Insert： | DNA: cDNA DESCRIPTION OF INSERT COMPONENT: Genome: Homo sapiens Gene symbol: SLC16A1 Gene name: solute carrier family 16 (monocarboxylate transporters), member 1 Contains complete coding sequence?: Y Chromosome: 1; Localization: 1 p13.2-p12 Tissue: heart Type of DNA: cDNA Insert 5 end: SalI Insert 3 end: PstI Insert size (kb): 2.5 Cross references: DNA Seq. Acc.: L31801 Insert lengths(kb): 2.5 Tissue: heart Gene product: mevalonate transporter(monocarboxylate transporter 1) SLC16A1 |
| Insert Size (kb)： | 2.5 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRV--4.1, 1.4; PstI--5.5; PstI/SalI--2.9, 2.6; XhoI--5.5; BamHI--5.5. The insert contains the following restriction sites (approximate kb from the 5 end): EcoRV--0.2, 1.6. Contains the complete coding sequence plus the 3 untranslated region of approximately 1 kb. |
| References： | Garcia CK, et al. cDNA cloning of the human monocarboxylate transporter 1 and chromosomal localization of the SLC16A1 locus to 1p13.2-p12. Genomics 23: 500-503, 1994. PubMed: 7835905 Joseph L Goldstein, personal communication |