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| 产品名称： | pLAFR1 cosmid in E. coli |
|---|---|
| 商品货号： | TS187562 |
| Designations： | pLAFR1 cosmid in E. coli |
| Depositors： | FM Ausubel |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 21.60 Vector: pLAFR1 (cosmid) Construction: pRK270, pHC79 Marker(s):tetR Construct size (kb): 21.60 Features: marker(s): tetR replicon: RK2, pMB1 |
| Applications： | shuttle vector vector for constructing genomic libraries |
| Comments： | IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + tet plates at 30C and picking small colonies for liquid culture. A broad host range cosmid vector containing a relaxation complex site. |
| Media： | ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline |
| Growth Conditions： | Temperature: 30.0°C |
| References： | Friedman AM, et al. Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants. Gene 18: 289-296, 1982. PubMed: 6290332 |
| Shipped： | freeze-dried |
| Shipping Information： | Shipped: xa0Freeze dried E. coli containing the cosmid |