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微信小章| 产品名称： | 28-6-20 |
|---|---|
| 商品货号： | TS187788 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma:lymphoblast B lymphocyte; somatic cell hybri |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The 28-2-7 (ATCC CRL-2487) and 28-6-20 (TS187788) antibodies bind the V kappa 24 light chain idiotypic specificity of MOPC167. MOPC167 is an IgA myeloma specific for PC and HPCM27 is an IgM myeloma specific for PC. The antibodies are specific for PC-binding proteins of VH1/V kappa 24 heavy and light chains of various isotypes. These antibodies can be used as typing reagents for mouse IgA bearing the V kappa 24 idiotypic specificity. They can be used in typing the 207-4 immunoglobulin transgenic mice that carry the VH1/V kappa 24 gene segment of the IgA PC specific MOPC167 myeloma. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against MOPC167 idiotype (V kappa 24) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against MOPC167 idiotype (V kappa 24) |
| Comments： | The 28-2-7 (ATCC CRL-2487) and 28-6-20 (TS187788) antibodies bind the V kappa 24 light chain idiotypic specificity of MOPC167. Animals were immunized with MOPC167 and HPCM27 anti-phosphocholine (PC) antibodies. MOPC167 is an IgA myeloma specific for PC and HPCM27 is an IgM myeloma specific for PC. Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibodies are specific for PC-binding proteins of VH1/V kappa 24 heavy and light chains of various isotypes. They do not bind VH1/V kappa 22, VH1/V kappa 8, or VH1/V kappa 1 PC-binding proteins or other IgA or IgM myeloma proteins. These antibodies can be used as typing reagents for mouse IgA bearing the V kappa 24 idiotypic specificity. They can be used in typing the 207-4 immunoglobulin transgenic mice that carry the VH1/V kappa 24 gene segment of the IgA PC specific MOPC167 myeloma. |
| Complete Growth Medium： | Iscoves modified Dulbeccos medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2xa0 x 105 viable cells/mL.xa0 Maintain cultures at a cell concentration between 5xa0 x 104 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL. Medium Renewal:xa0Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | rat IgG1 and 2a |
| Name of Depositor： | DG Sieckmann |
| Deposited As： | rat (B cell); mouse (myeloma) |
| Year of Origin： | 1990 |
| References： | Sieckmann DG, et al. Anti-idiotype monoclonal antibodies specific for the MOPC167 anti-phosphocholine transgene-encoded antibody. Hybridoma 16: 503-511, 1997. PubMed: 9455702 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |