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| 产品名称： | pBSL141 |
|---|---|
| 商品货号： | TS187820 |
| Designations： | pBSL141 |
| Depositors： | MF Alexeyev |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 3.8859999179840090 Vector: pBSL141 (phagemid) Promoters: Promoter for in vitro transcription T7 (phi10) Construction: pBluescript Marker(s):ampR,gtmR Construct size (kb): 3.885999917984009 Features: marker(s): ampR promoter for in vitro transcription: T7 (phi10) replicon: f1 replicon: pBM1 MCS: KpnI...SacI MluI MCS: MluI KpnI...SacI coding sequence: aacC1 (gtmR) |
| Applications： | contains easily purifiable cassette(s) for construction gentamicin acetyltransferase |
| Comments： | Restriction digests of the clone give the following sizes (kb): MluI--3.1, 0.8; BamHI--2.9, 1.0; NotI--2.9, 1.0. Problems have been reported when using DH5alpha as a host for multicopy plasmids bearing Gm and Tc, but not when TG1 is used. Plasmid useful for vector construction. Contains a gentamicin acetyltransferase (aacC1, gtmR) cassette flanked by multiple cloning sites duplicated in tandem orientation. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Alexeyev MF, et al. Improved antibiotic-resistance gene cassettes and omega elements for Escherichia coli vector contruction and in vitro deletion/insertion mutagenesis. Gene 160: 63-67, 1995. PubMed: 7628718 Mikhail F Alexeyev, personal communication |
| Shipped： | frozen |
| Shipping Information： | xa0Frozen glycerol stock of E. coli containing the phagemid |