微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pMSC1 |
|---|---|
| 商品货号： | TS188011 |
| Designations： | pMSC1 |
| Depositors： | NG Stoker |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli JM101 |
| Vector Information： | Size (kb): 13.1000003814697300 DESCRIPTION OF VECTOR: Intact vector size: 13.100 Type of vector: cosmid Cloning sites: BamHI HindIII NheI Polylinker sites: Cloning capacity: 30-42 kb Construction: Lawrist4, pYUB12 Host range: Mycobacterium sp.; Escherichia coli Features (with orientation and position when available): marker(s): ampR, kanR replicon: lambda, pMB1, pAL5000 terminator: trpA, rrnC, fd Vector: pMSC1 (cosmid) Construction: Lawrist4, pYUB12 Marker(s):ampR,kanR Construct size (kb): 13.10000038146973 Features: marker(s): ampR, kanR replicon: lambda, pMB1, pAL5000 terminator: trpA, rrnC, fd |
| Applications： | shuttle vector vector for constructing genomic libraries |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRV-8.2, 5.0; HindIII--13.5; BamHI--13.5; NheI--13.5; EcoRI--6.5, 2.5, 1.3. The pAL5000 ori permits replication in Mycobacterium smegmatis and M. bovis BCG. The vector should be selected using ampicillin and kanamycin in E. coli, and kanamycin in Mycobacterium spp. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. A cosmid shuttle vector useful for genomic DNA library construction. The ScaI site between the cos sites can be used to linearize the plasmid. After packaging, 10.3 kb of vector is retained, permitting an insert range of 30 - 42 kb. Sequences hybridizing to pBR322, including ampR, are removed. The order of the major features in this plasmid is: cos - pMB1 ori - ampR/ScaI - cos - trpA terminator - XbaI - HindIII - fd terminator - EcoRV - pAL5000 ori - BamHI - rrnC terminator - EcoRV - lambda ori - NheI - kanR. |
| Media： | ATCC Medium 1065 (see below) plus ampicillin (50.0 mcg/ml) plus kanamycin (20.0 mcg/ml)
ATCC Medium 1065:
Tryptone (Difco 0123), 10.0 g
Yeast Extract (Difco 0127), 5.0 g
NaCl, 10.0 g
Distilled water, 1.0 L
|
| Growth Conditions： | Temperature: 30.0°C |
| References： | Hinshelwood S, Stoker NG. An Escherichia coli-Mycobacterium shuttle cosmid vector, pMSC1. Gene 110: 115-118, 1992. PubMed: 1531970 Neil G Stoker, personal communication |
| Shipped： | frozen |
| Shipping Information： | Distributed: freeze-dried |