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微信小章| 产品名称： | lambdaEalphaTOU lambdaTOUepsilonalpha, lambdaepsilonalphaTOU |
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| 商品货号： | TS188052 |
| Designations： | lambdaEalphaTOU lambdaTOUepsilonalpha, lambdaepsilonalphaTOU |
| GenBank Number： | J00221 |
| Species： | Homo sapiens, human |
| Depositors： | M Lefranc, TH Rabbitts |
| Applications： | A 0.6 kb XhoI/PstI fragment has been used as an alpha-specific probe. |
| Vector： | Construct size (kb): 48.29999923706055 |
| Insert： | DNA: genomic Insert lengths(kb): 19.5 Gene product: immunoglobulin epsilon IGHE |
| Insert Size (kb)： | 19.5 |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried bacteria-free lysate |
| Comments： | Restriction digests of the clone give the following sizes (kb): BamHI--20.0, 16.0, 9.2, 2.8; EcoRI--20.0, 14.0, 9.2, 5.6; HindIII--23.0, 10.0, 7.8, 1.6; SacI--20.0, 9.2, 5.6, 4.6, 2.8, 2.5, 1.0 (doublet); XhoI--20.0, 14.0, 9.2, 5.0. The insert contains the following restriction sites (approximate kb from the 5 BamHI site): BamHI--2.8, 18.7; HindIII--9.5, 17.5; EcoRI--13.7. A 0.6 kb XhoI/PstI fragment has been used as an alpha-specific probe. The 3.8 kb EcoRI/HindIII fragment contains sequences characteristic of the A2m(2) allotype constant region and its 5 flanking sequences. The 1.1 kb HindIII/BamHI fragment contains the transmembrane exon. The insert begins just 5 to the sequences encoding the epsilon constant region and extend approximately 4.3 kb beyond the exons for the alpha chain. Additional GenBank/EMBL accession numbers: M35287, M35291. |
| References： | Lefranc MP, Rabbitts TH. Human immunoglobulin heavy chain A2 gene allotype determination by restriction fragment length polymorphism. Nucleic Acids Res. 12: 1303-1311, 1984. PubMed: 6322103 Bensmana M, Lefranc MP. Gene segments encoding membrane domains of the human immunoglobulin gamma 3 and alpha chains. Immunogenetics 32: 321-330, 1990. PubMed: 1979064 Flanagan JG, et al. Mechanisms of divergence and convergence of the human immunoglobulin alpha 1 and alpha 2 constant region gene sequences. Cell 36: 681-688, 1984. PubMed: 6421489 Bensmana M, et al. Sequence of the CH1 and hinge-CH2 exons of the human immunoglobulin IGHA2 A2m(2) allele: comparison with the nonallelic and allelic IGHA genes. Cytogenet. Cell Genet. 56: 128, 1991. PubMed: 1901541 Marie-Paule Lefranc, personal communication |