| 产品名称: | phgJE2-1 |
|---|---|
| 商品货号: | TS188128 |
| Designations: | phgJE2-1 |
| GenBank Number: | M26035 |
| Species: | Homo sapiens, human |
| Depositors: | BJ Rollins |
| Vector: | Construct size (kb): 5.199999809265137 |
| Insert: | DNA: genomic Insert lengths(kb): 2.200000047683716 Tissue: embryonic lung fibroblast WI-38 (serum-induced) Gene product: cytokine A2, small inducible( monocyte chemotactic protein, monocyte chemotactic protein 1, homologous to mouse Sig-je, small inducible cytokine A2, small inducible cytokine subfamily A (Cys-Cys), member 2, small inducible gene JE) JE* |
| Insert Size (kb): | 2.200 |
| Media: | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level: | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information: | Distributed: freeze-dried |
| Comments: | The insert contains the following restriction sites: BalI, PstI, SspI (2), BglI (2), SacI, NcoI, SpeI. Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.2; HindIII--5.2; PstI--4.3, 0.9; BglI--2.0 (doublet), 1.2; SalI/XbaI--3.1, 1.15, 0.5 (doublet). The 5 clone phgJE3-3 (ATCC 61366/61367) and the 3 clone phgJE2-1 (TS188128/61369) together generate a complete gene. The insert is oriented such that the EcoRI site in intron 1 is closer to the T7 promoter. |
| References: | Rollins BJ, et al. The human homolog of the JE gene encodes a monocyte secretory protein. Mol. Cell. Biol. 9: 4687-4695, 1989. PubMed: 2513477 Barrett J Rollins, personal communication |