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微信小章| 产品名称： | Cryptococcus amylolentus (van der Walt et al.) Golubev, anamorph |
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| 商品货号： | TS188151 |
| Deposited As： | Cryptococcus amylolentus (van der Walt et al.) Golubev |
| Strain Designations： | NRRL Y-7784 CBS 6039, IFO 10423, JCM 1690 |
| Application： | Multigene phylogeny and phenotypic characterization. |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | freeze-dried |
| Storage Conditions： | Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section |
| Type Strain： | yes |
| Preceptrol®： | no |
| Genome Sequenced Strain： | Yes |
| Comments： | utilizes D-xylose, xylitol and D-xylonate
Multigene phylogeny and phenotypic characterization.
Genome sequencing strain (Broad Institute, USA) |
| Morphology： | After 3 days on YM at 25°C, two colony types present on plates. One type off-white, smooth, mucoid, glistening; yeast cells subglobose or ovoid, single or in pairs, no pseudohyphae present. Second type white, raised, rough, dull; yeast cells subglobose or ovoid, single or in pairs, pseudohyphae present. |
| Medium： | ATCC® Medium 28: Emmons modification of Sabourauds agar ATCC® Medium 200: YM agar or YM broth ATCC® Medium 324: Malt extract agar |
| Growth Conditions： | Temperature: 24°C to 26°C
Atmosphere: Typical aerobic |
| Sequenced Data： |
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence AAGGATCAGTAGAGAATAACGGACTTCGGTCCATATATCTACCCATCTACACCTGTGAACCGTATTTGTGCTTCGGCACGTTTTACACAAACTTCTAAATGTAATGAATGTAAACTATTATAACAATAATAAAACTTTCAACAACGGATCTCTTGGCTTCCACATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCAACTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGAGTCATGAACATCTCAATCCTTCGGGTTTATGACCTGTTGGCATTGGATTTGGGTGTTTGCCGCGACCTGCAAAGGACGTCGGCTCGCCTTAAATGTGTTAGTGGGAAGGTGATTACCTGTCAGCCCGACGTAATAAGTTTCGTTGGGCCTATGGGGTAGTTGTCGGCTTGCTTATAACAACCATCTTTTTTGTTTGACCTCAAATCAGGTAGGGCTACCCGCTGAACTTAAG D1D2 region of the 28S ribosomal RNA gene CATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACGGCGAGTGAACCGGGAAGAGCTCAAATTTGAAATCTGGCGTCCTTCGGGCGTCCGAGTTGTAATCTACAGAAACGTTTTCCGTGCTGGACTGTGTCTAAGTTCCTTGGAATAGGATATCAAAGAGGGTGACAATCCCGTACTTGACACAATCACCAGTGCTCTGTGATACGTTTTCTACGAGTCGCGTTGCTTGGGAATGCAGCGCAAAATGGGTGGTAAACTCCATCTAAAGCTAAATATTGGTGGAAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAACGATTGAAGTCAGTCGTGTCTATTGGGTTCAACCAGTTCTGCTGGTGTATTCCCTTTAGACGGGTCAACATCAGTTCTGATCGGTGGATAAGGGCACGAGGAATGTAGCAATCTTCGGGTTGTGTTATAGCCTTGTGTCGCATACACTGGTTGGGACTGAGGAATGCAGCTCGCCTTTATGGCCGGGGTTCGCCCACGTTCGAGCTTAGGATGTTGACAAAATGGCTTTAAACGAC |
| Name of Depositor： | NRRL |
| Isolation： | Frass of the tunnels of Sinoxylon ruficorne infesting Dichrostachys cinerea near Groblersdal, Transvaal, South Africa |
| Cross References： | Nucleotide (GenBank) : AF105391 26S ribosomal RNA gene, partial sequence Nucleotide (GenBank) : AWGJ00000000 Cryptococcus amylolentus CBS 6039, whole genome shotgun sequencing project |
| References： | van der Walt JP, et al. Six new Candida species from South African insect sources. Mycopathol. Mycol. Appl. 47: 221-236, 1972. van der Walt JP, Steyn RL. Note on D-xylonate utilization by ascomycetous and basidiomycetous yeasts. Syst. Appl. Microbiol. 13: 192-193, 1990. Fell JW, et al. Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis. Int. J. Syst. Evol. Microbiol. 50: 1351-1371, 2000. PubMed: 10843082 Findley K, et al. Phylogeny and phenotypic characterization of pathogenic Cryptococcus species and closely related saprobic taxa in the Tremellales. Eukaryot Cell 8(3): 353-361, 2009. PubMed: 19151324 Guerreiro MA, et al. Molecular and genetic evidence for a tetrapolar mating system in the basidiomycetous yeast Kwoniella mangrovensis and two novel sibling species. Eukaryot Cell 12: 746-760, 2013. PubMed: 23524993 Findley K, et al. Discovery of a modified tetrapolar sexual cycle in Cryptococcus amylolentus and the evolution of MAT in the Cryptococcus species complex. PLoS Genet 8: e1002528, 2012. Scorzetti G, et al. Systematics of basidiomycetous yeasts: a comparison of large subunit D1/D2 and internal transcribed spacer rDNA regions. FEMS Yeast Res. 2: 495–517, 2002. PubMed: 12702266 Bovers M, et al. Six monophyletic lineages identified within Cryptococcus neoformans and Cryptococcus gattii by multi-locus sequence typing. Fungal Genet Biol 45: 400-421, 2008. PubMed: 18261945 |