微信小陈
微信小章
产品简介
购买须知
| 产品名称： | pBSL14 |
|---|---|
| 商品货号： | TS188156 |
| Designations： | pBSL14 |
| Depositors： | MF Alexeyev |
| Biosafety Level： | 1 |
| Host： | Distribution host: Escherichia coli HB101 (ATCC 33694) |
| Vector Information： | Size (kb): 3.9000000953674320 DESCRIPTION OF VECTOR COMPONENT: Name of vector: pUC18 Intact vector size: 2.686 Type of vector: cosmid Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI SmaI KpnI SacI EcoRI Construction: pUC71K Host range: Escherichia coli Features (with orientation and position when available): insert detection: lacZ, MCS: HindIII...EcoRI, -> promoter: lac, replicon: pMB1, marker(s): ampR, Cross references: DNA Seq. Acc.: L08752 Vector: pBSL14 (plasmid) Construction: pUC18, pK18 Marker(s):ampR,kanR Construct size (kb): 3.900000095367432 Features: marker(s): ampR marker(s): kanR replicon: pMB1 MCS: HindIII...EcoRI MCS: HindIII...NheI |
| Applications： | contains easily purifiable cassette(s) for construction aminoglycoside phosphotransferase kanamycin resistance, neomycin resistance |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.8, 1.1; HindIII--2.8, 1.1; BamHI--2.7, 1.2. The nptII gene was modified by site-directed mutagenesis to remove PstI and SphI sites. Plasmid with an easily purified kanR cassette (flanked by direct repeats of the pUC18 multiple cloning site) for use in construction. |
| Media： | ATCC® Medium 1948: LB medium (ATCC medium 1065) with 50 mcg/ml ampicillin and 20 mcg/ml kanamycin |
| Growth Conditions： | Temperature: 37.0°C |
| References： | Alexeyev MF. Three kanamycin resistance gene cassettes with different polylinkers. BioTechniques 18: 52-55, 1995. PubMed: 7702853 |
| Shipped： | freeze-dried |
| Shipping Information： | Distributed: freeze-dried |