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| 产品名称： | 49f p49f |
|---|---|
| 商品货号： | TS188166 |
| Designations： | 49f p49f |
| Species： | Homo sapiens, human |
| Depositors： | J Weissenbach |
| Applications： | For TaqI RFLPs, use low stringency (2X SSC, 42C). |
| Vector： | Construct size (kb): 7.159999847412109 |
| Insert： | DNA: genomic Insert lengths(kb): 2.799999952316284 Gene product: DNA Segment, single copy DYS1 Alleles: B2, D4, A5, B1, C2, D3, D4, F2, I2, D2, A1, A2, A3, A4, C1, D1, F1, I1 |
| Insert Size (kb)： | 2.800 |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information： | Distributed: freeze-dried |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--4.6, 3.0; HindIII--4.6, 1.7, 1.3; EcoRI/HindIII--4.6, 1.7, 1.3; BamHI--7.8; PstI--5.2, 2.7. EcoRI detects the cognate Y-specific band (2.8 kb) at high stringency (0.1X SSC, 68C); low stringency detects numerous (10-15) Y-specific bands and one 2 kb autosomal band. TaqI detects 5 independent allelic series representing at least 16 haplotypes, each defined by a different combination of the various forms of each of 5 RFLPs. For TaqI RFLPs, use low stringency (2X SSC, 42C). |
| References： | Ngo KY, et al. A DNA probe detecting multiple haplotypes of the human Y chromosome. Am. J. Hum. Genet. 38: 407-418, 1986. PubMed: 3010708 Lucotte G, et al. Y chromosome DNA polymorphisms in two African populations. Am. J. Hum. Genet. 45: 16-20, 1989. PubMed: 2568089 Jean Weissenbach, personal communication |