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| 产品名称： | pRS315 |
|---|---|
| 商品货号： | TS188202 |
| Designations： | pRS315 |
| Depositors： | P Hieter |
| Other IDs： | Nucleotide (GenBank) : U03441 Yeast centromere vector pRS315 with LEU2 marker, complete sequence |
| Biosafety Level： | 1 |
| Vector Information： | Size (kb): 6.018
Vector: pRS315 (phagemid)
Promoters: Promoter for in vitro transcription T7
Construction: pRSS56 pBluescript KS+, pBS(+)
Marker(s): LEU2,ampR
Construct size (kb): 6.018
Features: insert detection: lacZ marker(s): LEU2 marker(s): ampR promoter: lac promoter for in vitro transcription: T3 promoter for in vitro transcription: T7 replicon: ARSH4 replicon: f1 replicon: pMB1 MCS: SacI...KpnI centromere: CEN6 |
| Applications： | YC-type (centromeric) shuttle vector
Shuttle vector
Vector containing primer sites useful for sequencing
Vector permitting RNA synthesis in vitro
Vector permitting production of single-stranded DNA
Vector permitting visual detection of recombinants |
| Comments： | Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 2.1; BamHI--6.0; HindIII--6.0; KpnI--4.3, 1.7; KpnI/EcoRI--4.1, 1.5, 0.4. One of a series of pBluescript-based centromere vectors (ATCC 77142-77145, 77157-77158) differing in the yeast selectable marker gene. YC-type centromere vector permitting visual detection of recombinants and production of ssDNA in E. coli. Contains promoters for in vitro RNA synthesis, priming sites useful for sequencing, and encodes the lacZ alpha (lacZ) peptide. pRSS56, constructed by ligating a PvuI fragment (bp 498-2412) of pBluescript KS+ to a PvuI fragment (bp 2850-730) of pBS(+), contains the KS MCS from pBluescript KS+ and the unique NdeI and AatII sites between bla and f1 origin of pBS(+).
A fragment (2.235 kb) containing the LEU2 gene was inserted into the NdeI site and a cassette containing CEN6 and the ARS associated with histone 4 (ARSH4) was inserted into the AatII site of pRSS56. All ends were blunted. The order of the major features in this plasmid is: LEU2 - f1 ori (NaeI) - T7 promoter - lacZ/MCS - T3 promoter - pMB1 ori - bla - CEN6 - ARSH4. |
| Media： | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Growth Conditions： | Temperature: 37°C |
| References： | Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436 |
| Related Products： | Component of: ATCC 77190 |
| Shipped： | frozen |
| Shipping Information： | Frozen E. coli containing the phagemid |