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| 产品名称： | Chlamydia trachomatis Serovar I |
|---|---|
| 商品货号： | TS188293 |
| Classification： | Chlamydiacae |
| Deposited As： | Trachoma serotype i |
| Agent： | Chlamydia trachomatis Serovar I |
| Strain： | UW-12/Ur |
| Biosafety Level： | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format： | frozen 1 mL per vial |
| Storage Conditions： | -70°C or colder |
| Comments： | Note that activities with high potential for aerosol production require BSL 3 facilities and practices. Add glassbeads and vortex preparation to disrupt cells.xa0 Infect monolayer with disrupted material.xa0 Centrifuge at 3000 x rpm (750 x g) for 1 hour.xa0 Feed with fresh growth medium containing 1-2 µg/mL cycloheximide.xa0 Incubate at 37°C for 48 hours. Next-generation sequencing (NGS) at ATCC on the McCoy cell line (ATCC® CRL-1696™) used as the host has shown the presence of Mus Musculus mobilized endogenous polytropic provirus and Murine leukemia virus. |
| Effect on Host： | Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies or Giemsa. |
| Recommended Host： | McCoy (ATCC® CRL-1696™) |
| Growth Conditions： | Temperature: 36°C Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 90-100% confluent. Prior to infection, disrupt cells by sonication or vortex with sterile glass beads. Remove medium and inoculate with a small volume of inoculum (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 1). Adsorb by centrifugation at 1500 x g for 1 hour at 25°C. End adsorption by adding growth medium. Incubation: 1-3 days |
| Name of Depositor： | SP Wang |
| Source： | Male urethra with non-gonococcal urethritis |
| References： | Wang SP, et al. Three new immunologic types of trachoma-inclusion conjunctivitis organisms. J. Immunol. 110: 873-879, 1973. PubMed: 4631875 |